Protocols
General
Field Safety Protocol (DRAFT v1, Griffin-Nolan, 2021, developed at Syracuse University): [Word] [PDF]
Environmental sensors
HOW TO: Environmental sensors (Fridley Lab, 2021)
Environmental microcontroller units:
Version A (including aerial light and temp/RH sensors), by J. Stark & M. Htet (2019)
- Bill of materials ($30)
- Arduino code on GitHub and sensor data display R code
Version B (v3) (all components belowground), by J. Stark (rev 2021)
- Bill of materials ($20)
- Arduino code on GitHub and sensor data display R code
Soil moisture (resistance) probes: calibration by J. Stark (2021)
Version C "Silver Bullet" light sensors (with temp, RH, pressure), by J. Hamilton (2024)
Leaf function
Leaf-level photosynthesis, by J.M. Heberling (2013).
- light response curves: R code and sample data
- CO2 response curves: R code and sample data
Leaf construction cost, by J.M. Heberling (2011, revised 2017).
Epidermal microscopy (e.g., stomatal size, density), by A. Craddock and J. Fridley (2008).
Leaf lifespan via cohort sampling, by J. Fridley and J. Hamilton (2024).
Closed-chamber tissue respiration (particularly via the LI-850), by J. Martineau (2024).
Aboveground growth phenology
Spring foliar budbreak phenology, by J. Fridley and A. Craddock (2013).
Leaf chlorophyll monitoring, by J. Chieppa (2012).
Chemical analysis
Tissue griding via the large Wiley Mill, by J. Hamilton (2023).
C and N content: NC2100 autoanalyzer protocol, by A. Fox (2016), revised K. Martinez (2017).
Ash concentration in biomass, by E. Hinman and M. Ritchie (2017).
Chlorophyll a/b extractions, by K. Martinez (2017).
- Porra et al. 1989 reference [PDF]
Nonstructural carbohydrates (starch and sugars) in woody tissues, by Elise Hinman (2017).
Plant propagation
Softwood cuttings protocol, by A. Ebert (2018).